Hyphal Formation is Enhanced by 121°C Heat Inactivation of Serum Added to Culture Media

Main Article Content

Hong He
Xueting Wang
Congcong Li
Xiaotong Deng
Yan Fan
Jianxin Han

Abstract

Background: A novel method for inducing hyphae formation, was described.

Methods: Mycelia of experimental and clinical strains from 100 patients with oral lichen planus were cultured in RPMI 1640 medium with 56°C or 121°C inactivated calf sera. After these strains were cultured for 2 h to 7 days, the average hyphae formation rate was observed under light microscopy.

Results: The 121°C inactivated calf serum showed better outcomes than 56°C inactivated calf serum in terms of time and amounts of hyphae products.

Conclusions: For Candida albicans hyphae culture, the 121°C inactivated serum is superior to the 56°C inactivated serum.

Keywords:
Serum inactivation, Candida albicans, hyphae, yeast, oral lichen planus.

Article Details

How to Cite
He, H., Wang, X., Li, C., Deng, X., Fan, Y., & Han, J. (2020). Hyphal Formation is Enhanced by 121°C Heat Inactivation of Serum Added to Culture Media. Journal of Advances in Medicine and Medical Research, 32(6), 12-20. https://doi.org/10.9734/jammr/2020/v32i630427
Section
Original Research Article

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